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| Workshop description | Workshop DNA typing of bone samples will focus in details on all areas of DNA typing of bone samples, inc. specimen documentation, laboratory set-up, sample cleaning and preparation, removal of inhibitors, extraction techniques, mini-STR systems and evaluation of results plus the practical demonstration of sample preparation and DNA extraction hints for ancient material.
The number of participants is limited, therefore it is highly recommended to register as soon as possible.
The registration fee covers the 2-day intensive workshop on 4.-5. October, workshop materials, coffee-break refreshments and lunch as well as the social program on October 4th evening (Traditional Czech cuisine raut and beer tasting).
You will have a chance to see something very special while in Prague! National Technical Museum exhibits the Gregor Mendel’s manuscript on the inheritance of plant traits that was published under the title "Experiments in Plant Hybridization" in the Proceedings of the Natural Science Society of Brno. This founding publication was composed in Brno in 1865 and opened the door to the field of genetics.
| | | | Speakers | Invited speakers:
Bruce BUDOWLE (University of North Texas, USA)
Bruce will cover the procedures for difficult DNA templates, microbial forensics for pathogens on the skeletal remains, ancestry informative markers, post-extraction DNA storage and much more.
Phillip ENDICOTT (Musée de l'Homme, Paris, France)
Phillip will give his talk „Authenticating European ancient DNA haplotypes: lessons from the Tirolean Iceman“ and will also cover his latest findings in archaeogenetics.
Additional speakers:
Zoran Budimlija (Forensic DNA specialist at NYC Office of Chief Medical Examiner)
World Trade Center human identification project: experiences with individual body identification cases.
Novel forensic phenotypic DNA tests: eye and skin color predictors
Jan Cemper-Kiesslich (Department of Legal Medicine, University of Salzburg, Austria)
Quality assurance and data authentification in ancient DNA analysis
Daniel Vanek (Archaeogenetic laboratory, Forensic DNA Service, Czech Republic)
Process of Bone sample DNA typing step by step: comparison of procedures, hints and recommendations
Livia Zatkalikova (Department of Biology and DNA Analysis, Institute of Forensic Science, Slovak Republic)
DNA analysis of casework related skeletal human remains in Slovakia: laboratory setup, standard operating procedures and identification success rates
| | | | Workshop agenda | 3.10. (optional): Visit to the archaeological site (place to be decided)(not included in the registration fee, pre-registration required)
4.10. Non-DNA techniques: documentation (3D scan, CT scan, analyses of isotopes, special anthropology, facial reconstruction); sample preparation (practical demonstration of cleaning, cutting, and grinding (SPEX/Warring mill); DNA miniconference - case reports, round table discussion
5.10. DNA-techniques will cover extraction methods, removal of inhibitors, quantitation of aDNA extracts, microbial DNA in aDNA extracts, DNA phenotyping (eye/hair color, ABO blood groups), ancestry genotyping, evaluation of DNA typing results, cross-contamination controls, post-extraction DNA storage
6.10. (optional): trip to Ossuary Kutna Hora (www.kostnice.cz) )(not included in the registration fee, pre-registration required)
| | | | Collaborative exercise: | Participants of the workshop can participate in a COLLABORATIVE EXERCISE on DNA-BASED IDENTIFICATION OF BONE SAMPLES
The aim of the exercise is to make a progress on standardization and to identify the technical problems connected with in identification of bone samples. We will distribute only 1 sample set per participating laboratory. The number of sample sets is limited and will be distributed on the bases first-come-first-serve.
The labs will RECEIVE detailed step-by-step protocol but are encouraged to use their own procedure. The results of the collaborative exercise will be published. Certificates of successful completion will be issued to all laboratories.
Frequently asked questions about the COLLABORATIVE EXERCISE
| | | | Registration and fees: | The registration fee covers the 2-day intensive workshop on 4.-5. October, workshop materials, coffee-break refreshments, lunches and social program on October 4th evening.
Registration fee is 14910 CZK (590 EUR approx.) for regular participants, 12390 CZK (490 EUR approx.) for ISFG members and 10370 CZK (410 EUR approx.) for returning participants who attended the last workshop in 2008.
Due to many requests, registration and payment deadlines were extended to September 10, 2012.
IF YOU ARE UNABLE TO REACH EVEN THIS DEADLINE, PLEASE APPROACH DIRECTLY THE ORGANIZERS AT workshop2012@dna.com.cz
No refunds will be possible upon cancelation within and after this extended period.
Register on-line
All fees are payable in Czech Crowns (CZK) only. The preferred method of payment is by credit card. The payment system uses the most secure on-line system available and is accessible after your registration and subsequent login with your unique registration number and personal password.
However other methods like wire-transfer are also available. When using wire-transfer method please remit to:
Bank: Raiffeisenbank, a.s., Account #: 758544028/5500, IBAN: CZ0955000000000758544028, SWIFT: RZBCCZPP
Bank address: Raiffeisenbank, a.s, Hvezdova 1716/2b, 140 78 Praha 4, Czech Republic
Account holder: Forenzní DNA servis, s.r.o., Janovskeho 18, 170 00 Praha 7, Czech Republic
Address: Please add your registration number (115XXXXX) for your payment identification.
All bank transfer fees MUST be paid by participants (payment instruction OUR).
Cancelation and refund
Please note that your registration is binding. Cancelations need to be made in writing and will only be accepted by August 16, 2012. An administrative fee of CZK 1900 (EUR 73 approx.) will be deducted. Registration and other required fees will be due in full after the above deadline even in case of non-attendance.
| | | | Accommodation and travel information: | Prague is easily accessible by plane, train and car.
Please contact the virtual registration desk if you need a help with the accommodation.
| | | | Organizers | Institute of Legal Medicine
2nd Medical Faculty, Charles University in Prague
and
Forensic DNA Service
Archaeogenetic laboratory
Contact:
Phone: +420 233 931 123 or +420 731 503 250
E-mail: workshop2012@dna.com.cz
| | | | Technical support and registration: | Biologicals Ltd
| | | | Sponsors: |
| | | | References: |
- Vanek D., Silerova M., Urbanova V., Saskova L., Dubska J., Beran M. (2011) Genomic DNA extraction protocols for bone samples: The comparison of Qiagen and Zymo Research spin columns, Forensic Science International: Genetics Supplement Series, Volume 3, Issue 1, December 2011, Pages e397-e398
- Ehler E., Vanek D., Stenzl V., Vancata V. (2011) Y-chromosomal diversity of Valachs from the Czech Republic: Model for isolated population in Central Europe. Croatian Medical Journal Vol.52, No.3,p.358-367
- Vanek D, Saskova L, Koch H (2009) Kinship and Y-chromosome analysis of the 7th century human remains: novel DNA extraction and typing procedure for ancient material. Croatian Medical Journal Vol.50, No.3, August 2009
- Kubalek P, Stolz D, Saskova L, Vanek D. A Near Eastern haplotype in the burial of a young man with a fissure in the skull from the early medieval cemetery at Tetin [in Czech]. Archaelogy in Central Bohemia. 2008;1:645-50.
- Jon Davoren, Daniel Vanek, Rijad Konjhodzic, John Crews, Edwin Huffine, Thomas J.Parson (2007) Highly Effective DNA Extraction Method for Nuclear Short Tandem Repeat Testing of Skeletal Remains from Mass Graves. Croatian Medical Journal Vol.48, No.4, August 2007
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| | | | Workshop Venue | The Prague Bone workshop 2012 will take place at the STEPHOTEL , which is readily accesible by Prague Metro. MAP
You can stay either in this hotel or basically any other Prague hotel that is located close to the metro station.
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