HiTrap IgM Purification HP

HiTrap IgM Purification HP is a 1 ml column prepacked with Sepharose High Performancefor fast and efficient purification of monoclonal IgM.

• Optimized for fast, efficient purification of monoclonal IgM from hybridoma cell culture.
• Prepacked with a thiophilic adsorption medium, 2-mercaptopyridine coupled to Sepharose High Performance.
• Recovery of IgM is high with excellent activity retention.
• The top and bottom frits are manufactured from porous polyethylene
• Columns are delivered with a stopper on the inlet and a snap-off end on the outle

HiTrap IgM Purification HP is prepacked with a thiophilic adsorption medium, 2-mercaptopyridine coupled to Sepharose High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgM and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.