cDNA Synthesis Kit
- Biotechrabbit
- BR0400400
- Na skladě
- 1 664,00Kč
-
1 632,00Kč
Dostupné možnosti
Dopravné a balné
Transportní
podmínky
cena se připočítává k položkám v objednávce
mražené na suchém ledu (dry ice) 890 Kč bez DPH na zásilky v celkové ceně do 45 000 Kč bez DPH, nad tuto hodnotu je dopravné a balné zdarma
Features
- Highly efficient synthesis of long cDNAs (≥ 19 kb)
- Excellent yields at temperatures up to 60°C
- High sensitivity reverse transcription from low abundance template
- Superior performance in demanding applications,
including templates with a high degree of secondary structure
Applications
- First-strand cDNA synthesis for RT-PCR and qPCR
- Gene expression profiling
- RNA labeling and primer extension
- cDNA library construction
Catalog # Size Package Content BR0400401 10 rxn of 20 µl 10 µl Reverse Transcriptase
100 µl 5× cDNA Synthesis Buffer
20 µl dNTP Mix, 5 µl RNase Inhibitor
10 µl Hexamer and 5 µl Oligo (dT) Primer
1.5 ml PCR Grade WaterBR0400403 125 rxn of 20 µl 125 µl Reverse Transcriptase
1 ml 5× cDNA Synthesis Buffer
250 µl dNTP Mix, 62.5 µl RNase Inhibitor
125 µl Hexamer and 62.5 µl Oligo (dT) Primer
5 × 1.5 ml PCR Grade WaterBR0400404 250 rxn of 20 µl 2 × 125 µl Reverse Transcriptase
1 ml 5× cDNA Synthesis Buffer
2 × 250 µl dNTP Mix, 2 × 62.5 µl RNase Inhibitor
2 × 125 µl Hexamer
2 × 62.5 µl Oligo (dT) Primer
10 × 1.5 ml PCR Grade Waterbiotechrabbit™ cDNA Synthesis Kit provides superior components that ensure efficient first-strand cDNA synthesis from mRNA or total RNA templates. biotechrabbit Revert UP II Reverse Transcriptase, a proprietary MMuLV reverse transcriptase, carries point mutations in the polymerase and RNase H domains, eliminating RNase H activity and ensuring highly efficient reverse transcription with increased thermostability. biotechrabbit RNase Inhibitor is a potent non-competitive inhibitor of RNases.
The combination of highly efficient cDNA synthesis, effective RNase inhibition and outstandingly pure nucleotide allows high yields of cDNAs of more than 19 kb. For greater application flexibility, random hexamer primers, allowing all RNAs in the reaction to be used as templates, and an oligo (dT) primer, allowing synthesis of cDNA from only poly(A) tailed mRNA, are included.