Pfu DNA Polymerase, 2.5 U/µl
- Biotechrabbit
- BR0300100
- Na skladě
- 1 794,00Kč
-
1 760,00Kč
Dostupné možnosti
Dopravné a balné
Transportní
podmínky
cena se připočítává k položkám v objednávce
Dry ice 890 Kč bez DPH na zásilky v celkové ceně do 45 000 Kč bez DPH, nad tuto hodnotu je dopravné a balné zdarma
Features
- Accurate PCR for demanding applications
- About ten times higher accuracy than that of Taq DNA Polymerase
- High value for a fair price
Applications
- High Fidelity PCR
- Generation of PCR products for blunt cloning
- Site directed mutagenesis
Catalog #
Size
Package Content
BR0300101
100 U | 80 rxn
40 µl Pfu DNA Polymerase
500 µl 10× Pfu Reaction Buffer
500 µl 5× PCR EnhancerBR0300102
500 U | 400 rxn
200 µl Pfu DNA Polymerase
2 × 1.2 ml 10× Pfu Reaction Buffer
1.5 ml 5× PCR EnhancerThe biotechrabbit Pfu DNA Polymerase is a highly purified recombinant thermostable proofreading DNA polymerase. The enzyme is isolated from E. coli carrying a plasmid with the cloned Pyrococcus furiosus DNA polymerase gene.
Pfu DNA Polymerase has about ten times higher accuracy compared to Taq DNA Polymerase.
The enzyme catalyzes template-dependent nucleotides polymerization in the 5'-3' direction, additionally it exhibits 3'-5' exonuclease (proofreading) activity, what allows the correction of nucleotide incorporation errors.
The enzyme has no 5'-3' exonuclease activity and no detectable reverse transcriptase activity.
For the most demanding applications the supplied PCR additive 5x PCR Helper can be used. It is not necessary to use for every reaction, but in many cases it helps to achieve even better results with templates having high GC content and complex structures.
The use of dUTP and dITP is not recommended with Pfu, because Pfu binding to DNA templates with uracil and hypoxanthine stalls DNA synthesis.
Pfu DNA Polymerase produces blunt ended PCR products suitable for blunt cloning.