GenUP™ Blood RNA Kit

Features

• Fast and simple procedure
• High quality RNA isolated from fresh and frozen, EDTA- or citrate-treated blood
• Physical removal of DNA, no DNase treatment, no toxic β-mercaptoethanol

Applications

• Isolation of up to 8 µg total RNA from 0.5–1.0 ml whole blood

Purchase GenUP™ Blood RNA Kit

Catalog #	Size	Package Content
BR0701401	10 preps	Solutions, Mini Filters and Vials
BR0701402	50 preps	Solutions, Mini Filters and Vials
BR0701403	250 preps	Solutions, Mini Filters and Vials

biotechrabbit™ GenUP Blood RNA Kit is designed for fast isolation of total RNA from up to 1 ml whole blood from fresh or frozen samples that have been stabilized with EDTA or citrate. Erythrocytes are removed in an initial lysis step. After an second lysis step, genomic DNA is bound to a Mini Filter DNA, which can be discarded. RNA is selectively bound to a Mini Filter RNA, washed with two different buffers and eluted. Including lysis, isolated RNA is available in approximately 45 min. The isolated RNA is suitable for a wide range of different molecular biology applications, including RT-PCR. The GenUP Blood RNA Kit is designed for the use with blood. For other starting material, such as cell-free body fluids (including cerebrospinal fluid, serum, plasma or urine), tissue, stool samples, buffy coat, cultured or isolated cells, swabs, dried blood spots, viruses, fungi, bacteria or parasites, please refer to the GenUP Total RNA Kit (BR07009), GenUP Virus RNA Kit (BR07010), GenUP Virus DNA/RNA Kit (BR07011) or UPzol RNA Isolation Solution (BR07001). STARTING MATERIAL Fresh whole blood; stabilized with EDTA or citrate (0.5–1.0 ml) EXTRACTION TIME Approximately 45 min BINDING CAPACITY > 20 µg RNA TYPICAL YIELD Variable depending on the starting material; 1–8 µg RNA