ApStarTaq™ Hot-Start DNA Polymerase, 5 U/µl

Features

• Aptamer-based hot-start functionality
• No activation steps needed
• High PCR specificity and sensitivity
• Exceptionally pure Taq DNA Polymerase for sensitive PCR applications and high yields

Applications

• Fast PCR reactions in endpoint and real-time analysis
• Hot-start PCR up to 3 kb
• Amplification of low-copy-number targets
• RT-PCR and TA cloning

biotechrabbit ApStarTaq™ DNA Polymerase is an aptamer based hot-start enzyme and the first-choice for fast PCR reactions. The exceptional quality and purity of the enzyme ensures highest performance, suitable for standard and fast PCR cycling in both, endpoint and real-time assays. It ensures high product yields with low background and without primer–dimer formation or non-specific priming.

The aptamer binds to Taq DNA Polymerase and inhibits the enzyme activity at temperatures below 45°C. This ensures full hot-start functionality. The enzyme is released during standard PCR cycling conditions. There is no need for separate heating or denaturation steps, allowing fast PCR reactions.

 

Info: Recommended annealing temperature is 2°C above primer Tm (use gradient PCR to optimize the annealing temperature).

Catalog #	Size	Package Content
BR0201501	250 Units	50 µl ApStarTaq Hot-Start DNA Polymerase 1.8 ml 5X PCR Reaction Buffer 1.5 ml 50 mM MgCl2
BR0201502	1250 Units	250 µl ApStarTaq Hot-Start DNA Polymerase 3 × 1.8 ml 5X PCR Reaction Buffer 1.5 ml 50 mM MgCl2
BR0201503	5000 Units	4 Kits BR0201502 ApStarTaq Hot-Start DNA Polymerase, 5 U/µl (1250 U each)